Agilent 1260 Infinity HPLC  

Manufacturer: Agilent
Model: 1260
Agilent 1260 HPLC
Includes the Following Components
G1315D 1260 DAD VL
G1316A 1260 TCC
G1364C 1260 FC-AS
G1311B 1260 Quat Pump
G1329B 1260 ALS,

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Used Agilent 1260 LC System with Quaternary Pump and VWD detector  

This  Agilent 1260 LC System with Quaternary Pump and VWD detector included:
1260 Solvent Tray
1260 Quaternary Pump G1311C
1260 Autosampler G1329B
1260 Thermostatted Column Compartment
1260 VWD G1314F with standard Flow Cell
 

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Used Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD  

This Agilent 1260 HPLC included:
1260 Binary Pump SL and G4225A
1260 HiP Degasser with solvent tray  or G1311B(built-in degasser )
Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD),
 Agilent 1260
Autosampler (HiP-ALS SL)
 1260 Thermostat for Autosampler
If you are interested in Agilent 1260 HPLC with Thermostat for Autosampler , Binary Pump SL ,DAD,please contact us.

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Used Agilent 1260 HPLC with 1260 with Binary pump,DAD,Autosampler, Degasser with solvent tray  

This Agilent 1260 HPLC included:
Binary pump with switch valve
 1260 Binary Pump
 Thermostatted Column Compartment (TCC SL+)
 Diode Array Detector (DAD)
 Autosampler (HiP-ALS SL)
 1290 Thermostat for Autosampler
1260 HiP Degasser with solvent tray
 

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HPLC instrumentation and chromatography principle  

HPLC instrumentation and chromatography principle

This HPLC chromatography lecture explains the HPLC principle and instrumentation.
 
https://www.youtube.com/embed/pGYXd4k5F9o
 

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Used Agilent 1260 Infinity HPLC System with DAD,Quat Pump,ALS,TCC  

This Agilent 1290 Infinity Series HPLC System included:
1260: DAD
1260: Quat Pump (with built in Degasser); 600 Bar
1260: ALS
1260: TCC
W7 Pro Computer
Chemstation Software
 

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Agilent HPLC 1260  

The Agilent 1260 Infinity HPLC-Chip/MS system is a re-useable microfluidic chip-based technology for high sensitivity nanospray LC/MS. The Agilent 1260 replaced the Agilent 1200 and delivers results faster to save time and money. 

Agilent Technologies 1260 Series

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agilent 1260 hplc

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This Agilent 1260 HPLC included: Binary pump with switch valve 1260 Binary Pump Thermostatted Column Compartment (TCC SL+)

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Hplc Agilent Technologies 1260 Infinity II LC System Brochures  

Prove Solutions for Clnical Research and Forensic Toxicology
See Agilent's clinical research products and services for specific research applications.

16-Sep-2016
1.71 MB
PDF


Agilent InfinityLab. Maximize your LC Workflow Efficiency.
Agilent InfinityLab. Maximize your LC Workflow Efficiency.

15-Aug-2016
572.54 KB
PDF


Agilent InfinityLab. Maximize your LC workflow Efficiency.
Agilent InfinityLab. Maximize your LC workflow Efficiency.

26-Jul-2016
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Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.
Agilent InfinityLab. Maximum efficiency for your liquid chromatography workflows.

20-Jun-2016
4.26 MB
PDF


Agilent InfinityLab Supplies for the Agilent 1260 Infinity II LC with Multisampler
Agilent InfinityLab S

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Agilent HPLC  

Find great deals on HPLCHPLC.COM for Used and Refurbished  Agilent HPLC in Analytical Lab Instruments. Shop with confidence. 





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agilent 1200 hplc


hplc.hplchplc.com/list/agilent+1200+hplc.html
 
This HP/Agilent 1200 Series Nano HPLC system has just been moved from a working lab. It comes with the followings: G1379B Degasser with Tray G2226A ...
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Agilent Technologies 1260 Series


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USED AGILENT HP 1200 HPLC Series SYSTEM


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used

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1100 HPLC  

Introduced in 1995, Agilent's 1100 series HPLC system is considered the world's most popular HPLC. They are incredibly robust machines that can last a long time. Finding a used Agilent 1100 on LabX is easy and maintenance for older machines consists of changing seals, filter solvents, lamps, and the odd part in the vacuum degasser. Parts from the Agilent 1050 are often shared with the Agilent 1100 which make it easy to maintain.

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Agilent Technologies 1100 Series

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Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System. 1100 hplc,agilent hplc,hplc system,used hplc,used agilent hplc. HPLC System with ...

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Used Agilent 1100 S

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Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features Of Agilent 1260 Infinity II Variable Wavelength Detector:
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resoluti

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Agilent 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of versati

ادامه مطلب  

Hplc Agilent Technologies 1260 Infinity II LC System  

Infinitely more confident.
The new 1260 Infinity LC sets higher standards in performance and value to give you more confidence in your results. The 600 bar power range combines with 80 Hz UV detector speeds and up to 10 times higher sensitivity. That‘s true UHPLC performance! At the same time you can run your existing HPLC methods completely unchanged. In short, the best solution for any HPLC or UHPLC application.
And within the price range of a typical HPLC!


Raising the HPLC standard 

The 1260 Infinity LC raises the bar in HPLC! With a 600 bar power range up to 5 mL/min for isocratic, quaternary and binary systems  and 200 bar up to 10 mL/min for isocratic and quaternary systems – plus 80 Hz detector speeds and 10 x higher sensitivity, you will reach new levels of ve

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Agilent 1260 Infinity II Multiple Wavelength LC Detector  

Reliable, simultaneous quantification of primary compounds, by products and impurities
The diode array design of the Agilent 1260 Infinity II Multiple Wavelength Detector offers very low detector noise (less than ± 7 μAU) for precise quantification of trace levels, regardless of the number of signals recorded. Over a wavelength range from 190 to 950 nm, simultaneous detection of up to eight compound-specific wavelengths for optimum selectivity is provided. High-speed UV detection with up to 120 Hz data rates keeps pace with the analysis speed of fast LC.
Features:
Increased sensitivity and selectivity - with simultaneous acquisition of up to eight compound-specific wavelengths.
Low detection limits - low noise front-end electronics and special flow cell design deliver lowest detection

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Evaporative Light Scattering Detector (ELSD)  

An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC). It is commonly used for analysis of compounds where UV detection might be a restriction and therefore compounds does not very efficient absorb UV radiation, such as sugars, antivirals, antibiotics, lipids, phospholipids, terpenoids, and alcohols. ELSDs fall under the category of general-purpose detectors, similar to refractive index detectors (RI).

ELSD | Evaporative Light Scattering Detector

elsd.hplchplc.com/

 

... Scattering Detector. An evaporative light scattering detector (ELSD) is a detector used in conjunction with high-performance liquid chromatography (HPLC).

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P

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1290 HPLC  

The Agilent 1290 Infinity LC system provides the highest levels of speed, resolution, flexibility and sensitivity for any LC and LC/MS application. 

 

Used Agilent 1290 Infinity Series HPLC System

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1290 Infinity Series HPLC

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 Used Agilent 1290 Infinity Series HPLC System · Download Hplc Agilent Technologies1290 Infinity II LC System Brochure and Guide. Downloa

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HPLC Electro Chemical Detector ( ECD )  

There are several different types of ECs. The detection is based on amperometry, polarography, coulometry, and conductrometry. They offer high sensitivity, simplicity, convenience, and wide-spread applicability. It is especially suitable for the use with semi-micro or capillary type system.
Introduction Of Electro Chemical Detector ( ECD )
Electrochemical detection (ECD) for HPLC or UHPLC is an extremely selective and sensitive detection technique that is applied in a number of analyses such as the neurotransmitters dopamine, serotonin and noradrenalin. In combination with the proper electronics, ECD has an enormous linear dynamic range of more then 6 orders of magnitude. This means that concentrations can be measured as low as 50 pmole/L and as high as 100 µmol/L or more.
Fig. 1. HPLC w

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chromatography  

Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.

Chromatography

chromatography.hplchplc.com/ 
 







Chromatography is a physical method of separation that distributes components to separate between two phases, one stationary (stationary phase), the other (the mobile phase) moving in a definite direction. The eluate is the mobile phase leaving the column. The eluent is the solvent that carries the analyte.


Partition Chromatography


chromatography.hplchplc.com/page-461157.html
 
Partition chromatography was one of the first k

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AGILENT HP 1200 HPLC Series SYSTEM  

1200 Series HPLC System in excellent working condition.
Includes:
    •    Sold Guaranteed in Good Working Condition
    •    Tested and Verified in Good Working Condition prior to ship
•    G1322A Agilent 1200 Micro Vacuum Degasser
•    Your choice Binary or Quaternary Pump
•    Many AutoSamplers to choose from
•    G1316B Agilent 1200 Thermostatted Column Compartment
•    G1315D Agilent 1200/1260 DAD Diode Array Detector
•    Agilent Solvent tray and bottles
•    All fittings, cables, and tubing
•    Agilent Chemstation Software with License
•    Computer
•    21.5" LCD Monitor
•    Keyboard and mouse
    •    WARRANTY: 3 Months Parts and Labor
INSTALLATION on-site in USA.
Includes:
• Pump sea

ادامه مطلب  

Agilent 1260 Infinity II Variable Wavelength LC Detector  

Sensitive, fast and even more analyte information with dual wavelength capabilities
The Agilent 1260 Infinity II Variable Wavelength Detector offers lowest baseline noise and drift, resulting in lowest detection limits for robust quantification of trace level components. Even more sample information can be acquired in dual-wavelength mode. Time-programmable wavelength switching provides optimum sensitivity and selectivity for your applications.Highest productivity can be achieved with fast analysis at up to 120 Hz data rates.
Features :
Robust quantification of trace level components -lowest baseline noise and drift results in lowest detection limits.
More analyte information per run- withdual-wavelength capabilities.
High-resolution gainin fast LC -at up to 120 Hz data acquisition r

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USED AGILENT HP 1200 HPLC Series SYSTEM  

We offer an Agilent Technologies 1200 Series HPLC System in excellent working condition. Includes:
    •    Tested and Verified in Good Working Condition prior to ship
    •    G1322A Agilent 1200 Micro Vacuum Degasser
    •    Your choice Binary or Quaternary Pump
    •    Many AutoSamplers to choose from
    •    G1316B Agilent 1200 Thermostatted Column Compartment
    •    G1315D Agilent 1200/1260 DAD Diode Array Detector
    •    Agilent Solvent tray and bottles
    •    All fittings, cables, an

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principle of hplc:High Performance Liquid Chromatography (HPLC)  

High performance liquid chromatography (HPLC) has become a very versatile and powerful separation and analytical method over the years. It is an advanced form of liquid chromatography (LC).
Instead of introducing the solvent into the column and allowing it to drip down under the influence of gravity, in HPLC the sample is forced through the column under high pressures of nearly 400 atm, resulting in faster and more efficient separation.
This technique is also called high pressure liquid chromatography.

The Principle of HPLC
HPLC follows the same basic principle as chromatography. Different components in the sample have varying affinities to the adsorbent material. This causes a difference in the flow rate for each component which leads to their separation as they come out of the column.

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column chromatography principle  

When a mixture of mobile phase and sample to be separated are introduced from top of the column, the individual components of mixture move with different rates.Those with lower affinity and adsorption to stationary phase move faster and eluted out first while those with greater adsorption affinity move or travel slower and get eluted out last.
The solute molecules adsorb to the column in a reversible manner. The rate of the movement of the components is given as follows
R= Rate of movement of a component / Rate of movement of mobile phase. i.e. it is the ratio of distance moved by solute to the distance moved by solvent.
source:studyread.com/

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Column Chromatography is the the first form of techniques developed in chromatography!  

Column chromatography is the prototype or the basic type of chromatography. It was the first form of techniques developed in chromatography. One can easily explain the principle and procedure of chromatography using it.
Other types of chromatography techniques like high performance liquid chromatography (HPLC), gas chromatography (GC), paper chromatography were developed with column chromatography as a module and making slight variations.
In-spite of many advanced methods of chromatography, still this method of chromatography is widely used in research and industry.
This chromatography is basically a type of adsorption chromatography techniques.

Here the separation of components depends upon the extent of adsorption to stationary phase. Here the stationary phase is a polar solid materia

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Principle of HPLC:High Performance Liquid Chromatography (HPLC) : Principle, Types, Instrumentation and Applications  

Chromatography is a technique to separate mixtures of substances into their components on the basis of their molecular structure and molecular composition. This involves a stationary phase (a solid, or a liquid supported on a solid) and a mobile phase (a liquid or a gas). The mobile phase flows through the stationary phase and carries the components of the mixture with it. Sample components that display stronger interactions with the stationary phase will move more slowly through the column than components with weaker interactions. This difference in rates cause the separation of variuos components. Chromatographic separations can be carried out using a variety of stationary phases, including immobilized silica on glass plates (thin-layer chromatography), volatile gases (gas chromatography

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Displacement Chromatography  

The basic principle of displacement chromatography is: A molecule with a high affinity for the chromatography matrix (the displacer) will compete effectively for binding sites, and thus displace all molecules with lesser affinities. There are distinct differences between displacement and elution chromatography. In elution mode, substances typically emerge from a column in narrow, Gaussian peaks. Wide separation of peaks, preferably to baseline, is desired in order to achieve maximum purification. The speed at which any component of a mixture travels down the column in elution mode depends on many factors. But for two substances to travel at different speeds, and thereby be resolved, there must be substantial differences in some interaction between the biomolecules and the chromatography m

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Principle Of Column Chromatography  

Column chromatography in chemistry is a method used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. The main advantage of column chromatography is the relatively low cost and disposability of the stationary phase used in the process. The latter prevents cross-contamination and stationary phase degradation due to recycling.
The classical preparative chromatography column is a glass tube with a diameter from 5 mm to 50 mm and a height of 5 cm to 1 m with a tap and some kind of a filter (a glass frit or glass wool plug – to prevent the loss of the stationary phase) at the bottom. Two methods are generally used to prepare a column: the dry method and the wet method.

For the dry

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Partition Chromatography  

Partition chromatography was one of the first kinds of chromatography that chemists developed. The partition coefficient principle has been applied in paper chromatography,thin layer chromatography, gas phase and liquid–liquid separation applications. The 1952Nobel Prize in chemistry was earned by Archer John Porter Martin and Richard Laurence Millington Synge for their development of the technique, which was used for their separation of amino acids. Partition chromatography uses a retained solvent, on the surface or within the grains or fibers of an "inert" solid supporting matrix as with paper chromatography; or takes advantage of some coulombic and/or hydrogen donor interaction with the stationary phase. Analyte molecules partition between a liquid stationary phase and the eluent.

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waters hplc  

Whatever your application, Alliance® HPLC, BreezeTM 2 HPLC, and AutoPurificationTM systems offer you proven solutions for all of your HPLC requirements - for today and the future. These systems are synonymous with dependable, routine performance and versatility.

Alliance HPLC Providing versatile, dependable performance for analytical labs, the Alliance HPLC family meets the rigorous requirements of routine chromatography and the performance standards of new-product research and method development.

Breeze 2 HPLC Comes with a pump, detector, injector, and is pre-configured for different levels of HPLC. It includes Breeze Software, an intuitive interface that is easy to set-up, learn, and use.
waters


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Waters Alliance HPLC THAT WORKS AS HARD AS YOU

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Shimadzu Evaporative Light Scattering LC Detector LTII (ELSD-LTII)  

In the history of high-performance liquid chromatographs, which dates to the early 1960s, refractive index detectors (RI detectors) have often been used as general-purpose detectors. RI detectors enable the detection of components that do not possess UV absorbance and give a proportional relationship between the heights of detected peaks and the quantities of detected components. So, in comparison with absorbance detectors (UV detectors), they offer advantages such as the ability to ascertain unknown component quantities and obtain molecular weight distributions for macromolecules. On the other hand, they also have various disadvantages. For example, they cannot be used for gradient analysis, the baselines they produce are susceptible to the influence of fluctuations in the ambie

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USED Agilent 1200 Series HPLC system with Diode Array Detector & Degasser  

This used  HP / Agilent 1200 Series HPLC system comes with the followings:


Solvent Tray


Agilent 1200 Series G1379B Degasser


Agilent 1200 Series G1312A Bin Pump


Agilent 1200 G1367B HiP ALS Autosampler


Agilent 1200 G1330B FC/ALSTherm


Agilent 1200 Series G1316A TCC 


Agilent 1200 Series G1315B DAD 


PC with Suitable software and all essential cables required for full operation


Fore more information, about used Agilent 1200 Series HPLC system with Diode Array Detector & Degasser please call.

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 Used Agilent 1200 series with Micro Degasser ,Quatenary Pump , Autosampler , VWD  

Whatever your liquid chromatography analysis needs are you will find your individual solution within the Agilent LC portfolio. The Agilent 1200 Series is the industry-leading standard, offering everything you need for analytical HPLC methodologies. From routine analysis through new product research and development in chemical analysis and pharmaceutical industries, the Agilent 1200 Series helps you achieve reliable and robust results for all your applications.

 LC system options:


Agilent 1200 series G1379B Micro Degasser
Agilent 1200 series G1311A Quatenary Pump
Agilent 1200 series G1329A Autosampler
Agilent 1200 series G1330B ALS Therm
Agilent 1200 series G1316A  Infinity Thermostatted Column Compartment
Agilent 1200 series G1314 Variable Wavelength Detector (VWD)

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Agilent Products  

Agilent Artisan Link for Special Stains
Agilent Atomic Absorption
Agilent Atomic Spectroscopy
Agilent Automated Electrophoresis
Agilent Automation Solutions
Agilent Autostainer Link Solution for IHC
Agilent Autostainer Plus for IHC
Agilent Bioanalyzer & TapeStation Solutions
Agilent BioMolecule Synthesis & Purification
Agilent Capillary Electrophoresis & CE/MS
Agilent Cell Analysis (Seahorse)
Agilent CGH & CGH+SNP Microarrays
Agilent CRISPR/CAS
Agilent Dako CoverStainer for H&E
Agilent Dako Omnis Solution for IHC & ISH
Agilent Diagnostic Services
Agilent Dissolution
Agilent Epigenetic & Specialty Microarrays
Agilent FISH Probes
Agilent Flow Cytometry
Agilent Fluorescence
Agilent FTIR
Agilent Gas Chromatography
Agilen

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Reversed Phase Chromatography (RPC)  

Reversed phase HPLC (RP-HPLC) has a non-polar stationary phase and an aqueous, moderately polar mobile phase. One common stationary phase is a silica which has been surface-modified with RMe2SiCl, where R is a straight chain alkyl group such as C18H37 or C8H17. With such stationary phases, retention time is longer for molecules which are less polar, while polar molecules elute more readily (early in the analysis). An investigator can increase retention times by adding more water to the mobile phase; thereby making the affinity of the hydrophobic analyte for the hydrophobic stationary phase stronger relative to the now more hydrophilic mobile phase. Similarly, an investigator can decrease retention time by adding more organic solvent to the eluent. RP-HPLC is so commonly used that it is oft

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principle of hplc:How Does High Performance Liquid Chromatography Work?/what is Detector,Chromatogram?  

How Does High Performance Liquid Chromatography Work?

The components of a basic high-performance liquid chromatography [HPLC] system are shown in the simple diagram in Figure E.
A reservoir holds the solvent [called the mobile phase, because it moves]. A high-pressure pump [solvent delivery system or solvent manager] is used to generate and meter a specified flow rate of mobile phase, typically milliliters per minute. An injector [sample manager or autosampler] is able to introduce [inject] the sample into the continuously flowing mobile phase stream that carries the sample into the HPLC column. The column contains the chromatographic packing material needed to effect the separation. This packing material is called the stationary phase because it is held in place by the column hardware.

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Used Agilent 1100 Series HPLC with Multiple Wavelength Detector System  

HPLC System with Computer
Agilent 1200 HPLC system includes,


G1315B DAD Detector


G1316A Colcom column compartment 


G1329A ALS Auto sampler 


G1311A Quat Pump 


 G1322A Degasser 


G1314D VWD Detector 


G4208A Controller 


Tray, Computer, Monitor, Column rack and Documentation


Agilent 1200 HPLC Complete System with Computer​  is currently not available.

 
s

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